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Prof.dr.ir. P.J.G.M. de Wit Person

Prof.dr.ir. P.J.G.M. de Wit

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  • Affinity of Avr2 for tomato cysteine protease Rcr3 correlates with the Avr2-triggered Cf-2-mediated hypersensitive response (2011)

    Authors:Klooster, van t J.W.; Kamp, van der M.W.; Vervoort, J.J.M.; Beekwilder, J.; Boeren, S.; Joosten, M.H.A.J.; Thomma, B.P.H.J.; Wit, de P.J.G.M.
    In:Molecular Plant Pathology, 12(1), 21 - 30

    Abstract:

    The Cladosporium fulvum Avr2 effector is a novel type of cysteine protease inhibitor with eight cysteine residues that are all involved in disulphide bonds. We have produced wild-type Avr2 protein in Pichia pastoris and determined its disulphide bond pattern. By site-directed mutagenesis of all eigh

  • Schimmels zijn genetische wildebrassen (interview met Gert Kema) (2011)Open access

    Authors:Mehrabi, R.; Wit, de P.; Kema, G.H.J.
    In:Gewasbescherming, 42(2 (april 2011)), Nieuws - 95. ISSN 0166-6495.

    Abstract:

    Pathogene schimmels doen spontaan waar organisaties als Greenpeace van gruwen: ze wisselen genen of zelfs hele chromosomen uit met andere schimmelsoorten. Dat blijkt uit een artikel in FEMS Microbiology Reviews van de Wageningse genetici Rahim Mehrabi, Pierre de Wit en Gert Kema van Wageningen UR.

  • Horizontal gene and chromosome transfer in plantpathogenic fungi affecting host range (2011)

    Authors:Mehrabi, R.; Bahkali, A.H.; Abd-Elsalam, K.A.; Ben M'Barek, S.; Mirzadi Gohari, A.; Karimi Jashini, M.; Stergiopoulos, I.; Kema, G.H.J.; Wit, de P.J.G.M.
    In:FEMS Microbiology Reviews. ISSN 0168-6445.

    Abstract:

    Plant pathogenic fungi adapt quickly to changing environments including overcoming plant disease resistance genes. This is usually achieved by mutations in single effector genes of the pathogens, enabling them to avoid recognition by the host plant. In addition, horizontal gene transfer (HGT) and ho

  • Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola Reveals Dispensome Structure, Chromosome Plasticity, and Stealth Pathogenesis (2011)Open access

    Authors:Goodwin, S.B.; Ben M'Barek, S.; Dhillon, B.; Wittenberg, A.H.J.; Crane, C.F.; Hane, J.K.; Foster, A.J.; Lee, van der T.A.J.; Grimwood, J.; Aerts, A.; Antoniw, J.; Bailey, A.; Bluhm, B.; Bowler, J.; Bristow, J.; Burgt, van der A.; Canto-Canché, B.; Churchill, A.C.L.; Conde-Ferràez, L.; Cools, H.J.; Coutinho, P.M.; Csukai, M.; Dehal, P.; Wit, de P.; Donzelli, B.; Geest, van de H.C.; Ham, van R.C.H.J.; Hammond-Kosack, K.E.; Henrissat, B.; Kilian, A.; Kobayashi, A.K.; Koopmann, E.; Kourmpetis, Y.; Kuzniar, A.; Lindquist, E.; Lombard, V.; Maliepaard, C.; Martins, N.; Mehrabi, A.; Nap, J.P.H.; Ponomarenko, A.; Rudd, J.J.; Salamov, A.; Schmutz, J.; Schouten, H.J.; Shapiro, H.; Stergiopoulos, I.; Torriani, S.F.F.; Tu, H.; Vries, de R.P.; Waalwijk, C.; Ware, S.B.; Wiebenga, A.; Zwiers, L.H.; Oliver, R.P.; Grigoriev, I.V.; Kema, G.H.J.
    In:Plos Genetics, 7(6). ISSN 1553-7390.

    Abstract:

    Abstract The plant-pathogenic fungus Mycosphaerella graminicola (asexual stage: Septoria tritici) causes septoria tritici blotch, a disease that greatly reduces the yield and quality of wheat. This disease is economically important in most wheat-growing areas worldwide and threatens global food pr

  • Afstandsonderwijs plantenziektekunde en plantenveredeling Ontwikkeling en eerste ervaringen (2011)Open access

    Authors:Goud, J.C.; Smant, G.; Niks, R.E.; Loon, van J.J.A.; Lent, van J.W.M.; Thiewes, H.; Wit, de P.J.G.M.
    In:Gewasbescherming, 42(4), 179 - 179. ISSN 0166-6495.

  • Schimmels variëren erop los (interview met P. de Wit) (2011)Open access

    Authors:Scharroo, J.; Wit, de P.J.G.M.
    In:Bionieuws, 21. ISSN 0924-7734.

  • Detection of Mycosphaerella graminicola in Wheat Leaves by a Microsatellite Dinucleotide Specific-Primer (2011)Open access

    Authors:Abd-Elsalam, K.; Bahkali, A.H.; Moslem, M.; Wit, de P.J.G.M.; Verreet, J.A.
    In:International Journal of Molecular Sciences, 12(1), 682 - 693. ISSN 1422-0067.

    Abstract:

    Early detection of infection is very important for efficient management of Mycosphaerella graminicola leaf blotch. To monitor and quantify the occurrence of this fungus during the growing season, a diagnostic method based on real-time PCR was developed. Standard and real-time PCR assays were develop

  • EBR1, a novel Zn2Cys6 transcription factor, affects virulence and apical dominance of hyphal tip in Fusarium graminearum (2011)

    Authors:Zhao, Cunzhao; Waalwijk, C.; Wit, de P.; Lee, van der T.A.J.; Tang, D.
    In:Molecular Plant-Microbe Interactions, 24(12), 1407 - 1418. ISSN 0894-0282.

    Abstract:

    Zn2Cys6 transcription factors are unique to fungi and have been reported to be involved in different regulatory functions. Here, we characterized EBR1 (enhanced branching 1), a novel Zn2Cys6 transcription factor of Fusarium graminearum. Knocking out EBR1 in F. graminearum PH-1 caused reduction of bo

  • Tomato Cf resistance proteins mediate recognition of cognate homologous effectors from fungi pathogenic on diots and monocots (2010)

    Authors:Stergiopoulos, I.; Burg, van den H.A.; Ökmen, B.; Beenen, H.G.; Liere, van S.; Kema, G.H.J.; Wit, de P.J.G.M.
    In:Proceedings of the National Academy of Sciences of the United States of America, 107(16), 7610 - 7615

    Abstract:

    Most fungal effectors characterized so far are species-specific and facilitate virulence on a particular host plant. During infection of its host tomato, Cladosporium fulvum secretes effectors that function as virulence factors in the absence of cognate Cf resistance proteins and induce effector-tri

  • Culture collections, the new herbaria for fungal pathogens (2010)

    Authors:Abd-Elsalam, K.A.; Yassin, M.A.; Moslem, M.A.; Bahkali, A.H.; Wit, de P.J.G.M.; McKenzie, E.H.C.; Stephenson, S.L.; Cai, L.; Hyde, K.D.
    In:Fungal Diversity, 45(1), 21 - 32

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