Prof.dr. H.A. Verbrugh

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Erasmus University Rotterdam (92)

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Rapid detection of methicillin resistance in Staphylococcus aureus isolates by the MRSA-screen latex agglutination test (1999)Open access

Authors:	Leeuwen, van W.B. (Willem); Pelt, van C. (Cindy); Luijendijk, A. (Ad); Verbrugh, H.A. (Henri); Goessens, W.H.F. (Wil)
In:	Journal of Clinical Microbiology. ISSN 0095-1137.

Abstract:

The slide agglutination test MRSA-Screen (Denka Seiken Co., Niigata, Japan) was compared with the mecA PCR ("gold standard") for the detection of methicillin resistance in Staphylococcus aureus. The MRSA-Screen test detected the penicillin-binding protein 2a (PBP2a) ant

Follow-up of Staphylococcus aureus nasal carriage after 8 years: redefining the persistent carrier state (1999)Open access

Authors:	Bergh, van den M.F.Q. (Marjolein); Yzerman, E.P.; Belkum, van A.F. (Alex); Boelens, H.A.M. (Hélène); Sijmons, M.; Verbrugh, H.A. (Henri)
In:	Journal of Clinical Microbiology. ISSN 0095-1137.

Abstract:

Studies of Staphylococcus aureus nasal carriage have distinguished three carriage patterns: persistent, intermittent, and noncarriage. The criteria used to identify these carriage patterns have been inconsistent. In 1988 the S. aureus nasal carrier index, i.e., the prop

The prevalence and clonal expansion of high-level gentamicin-resistant enterococci isolated from blood cultures in a Dutch university hospital (1999)Open access

Authors:	Braak, van den N.P.W.C.J. (Nicole); Belkum, van A.F. (Alex); Kreft, D.; Witt, te R. (René); Verbrugh, H.A. (Henri); Endtz, H.P. (Hubert)
In:	Journal of Antimicrobial Chemotherapy. ISSN 0305-7453.

Abstract:

We studied the prevalence and clonality of high-level gentamicin-resistant enterococci (HLGRE) in a Dutch university hospital. Of 238 enterococcal strains isolated from blood cultures between 1991 and 1997, 57 were HLGRE. Genomic analysis of these strains revealed 19 di

Persistence of clones of coagulase-negative staphylococci among premature neonates in neonatal intensive care units: two-center study of bacterial genotyping and patient risk factors (1998)Open access

Authors:	Vermont, C.L.; Hartwig, N.G. (Nico); Fleer, A.; Man, de P. (Peter); Groot, de R. (Ronald); Belkum, van A.F. (Alex); Verbrugh, H.A. (Henri); Anker, van den J.N. (John)
In:	Journal of Clinical Microbiology. ISSN 0095-1137.

Abstract:

From 1 January 1995 until 1 January 1996, we studied the molecular epidemiology of blood isolates of coagulase-negative staphylococci (CoNS) in the Neonatal Intensive Care Units (NICUs) of the Sophia Children's Hospital (SCH; Rotterdam, The Netherlands) and the Wilhelmina Children'

Comparison of eight methods to detect vancomycin resistance in enterococci (1998)Open access

Authors:	Endtz, H.P. (Hubert); Braak, van den N.P.W.C.J. (Nicole); Belkum, van A.F. (Alex); Goessens, W.H.F. (Wil); Kreft, D.; Stroebel, A.B.; Verbrugh, H.A. (Henri)
In:	Journal of Clinical Microbiology. ISSN 0095-1137.

Abstract:

A collection of genetically unrelated vancomycin-resistant enterococci (VRE) including 50 vanA, 15 vanB, 50 vanC1, and 30 vanC2 VRE were used to evaluate the accuracy of eight currently available susceptibility test methods (agar dilution, disk diffusion, E-test, agar s

Comparison of pharmacodynamics of azithromycin and erythromycin in vitro and in vivo (1998)Open access

Authors:	Hollander, den J.G. (Jan); Knudsen, J.D.; Mouton, J.W. (Johan); Fuursted, K.; Frimodt-Moller, N.; Verbrugh, H.A. (Henri); Espersen, F.
In:	Antimicrobial Agents and Chemotherapy. ISSN 0066-4804.

Abstract:

In this study, we determined the efficacy of various dosing regimens for erythromycin and azithromycin against four pneumococci with different susceptibilities to penicillin in an in vitro pharmacokinetic model and in a mouse peritonitis model. The MIC was 0.03 microg/m

Use of pharmacodynamic parameters to predict efficacy of combination therapy by using fractional inhibitory concentration kinetics (1998)Open access

Authors: Hollander, den J.G. (Jan); Mouton, J.W. (Johan); Verbrugh, H.A. (Henri)
In: Antimicrobial Agents and Chemotherapy. ISSN 0066-4804.

Abstract:
Combination therapy with antimicrobial agents can be used against bacteria that have reduced susceptibilities to single agents. We studied various tobramycin and ceftazidime dosing regimens against four resistant Pseudomonas aeruginosa strains in an in vitro pharmacokin
Duration and clinical relevance of postantibiotic effect in relation to the dosing interval (1998)Open access

Authors: Hollander, den J.G. (Jan); Fuursted, K.; Verbrugh, H.A. (Henri); Mouton, J.W. (Johan)
In: Antimicrobial Agents and Chemotherapy. ISSN 0066-4804.

Abstract:
The influence of half-life on the postantibiotic effect (PAE) of tobramycin against Pseudomonas aeruginosa and Staphylococcus aureus was investigated during one dosing interval. Tobramycin half-lives of 0.5 to 2.5 h were simulated in an in vitro model, and the PAE was d
Short-sequence DNA repeats in prokaryotic genomes (1998)Open access

Authors: Belkum, van A.F. (Alex); Scherer, S.; Alphen, van L. (Loek); Verbrugh, H.A. (Henri)
In: Microbiology and Molecular Biology Reviews. ISSN 1092-2172.

Abstract:
Short-sequence DNA repeat (SSR) loci can be identified in all eukaryotic and many prokaryotic genomes. These loci harbor short or long stretches of repeated nucleotide sequence motifs. DNA sequence motifs in a single locus can be identical and/or heterogeneous. SSRs are

Authors:	Hollander, den J.G. (Jan); Mouton, J.W. (Johan); Verbrugh, H.A. (Henri)
In:	Antimicrobial Agents and Chemotherapy. ISSN 0066-4804.

Authors:	Hollander, den J.G. (Jan); Fuursted, K.; Verbrugh, H.A. (Henri); Mouton, J.W. (Johan)
In:	Antimicrobial Agents and Chemotherapy. ISSN 0066-4804.

Authors:	Belkum, van A.F. (Alex); Scherer, S.; Alphen, van L. (Loek); Verbrugh, H.A. (Henri)
In:	Microbiology and Molecular Biology Reviews. ISSN 1092-2172.

Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study (1998)Open access

Authors:	Belkum, van A.F. (Alex); Salmenlinna, S.; Kooistra, M.; Cookson, B. (Barry); Witte, W.; El Solh, N.; Forey, F.; Etienne, J. (Jerome); Goering, R.; Morvan, A.; Struelens, M.; Vuopio-Varkila, J.; Tenover, F.C. (Fred); Steward, C.; Legakis, N.; Talens, A.; O'Brien, F. (Frances); Tassios, P.; Ryck, de R.; Grubb, W.; Kaufmann, M.E.; Verbrugh, H.A. (Henri); Leeuwen, van W.B. (Willem)
In:	Journal of Clinical Microbiology. ISSN 0095-1137.

Abstract:

Twenty well-characterized isolates of methicillin-resistant Staphylococcus aureus were used to study the optimal resolution and interlaboratory reproducibility of pulsed-field gel electrophoresis (PFGE) of DNA macrorestriction fragments. Five identical isolates (one PFG

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