Typing data obtained by specifically targeting a single, high-stringency
PCR at the pneumococcal BOX repeat element for 28 strains of Streptococcus
pneumoniae completely corroborated the resolutions attained by five
genotypic procedures as described by Hermans et al. (P.W.M. Hermans, M.
Sluijter, T. Hoogenboezem, H. Heersma, A. van Belkum, and R. de Groot, J.
Clin. Microbiol. 33:1606-1612, 1995). All pairs of strains, except one,
derived from both the cerebrospinal fluid and blood of the same individual
were shown to be identical. Moreover, other, epidemiologically unrelated
isolates were demonstrated to be unique. Considering the combined data
from the five typing techniques applied previously as the "gold standard,"
the single BOX PCR test demonstrated excellent resolving powers while
maintaining epidemiological linkage.