| Title |
Comparison of five tests for identification of Staphylococcus aureus from clinical samples |
| Published in |
Journal of Clinical Microbiology. ISSN 0095-1137. |
| Author |
Luijendijk, A. (Ad); Belkum, van A.F. (Alex); Kluytmans, J.A.J.W.; Verbrugh, H.A. (Henri) |
| Date |
1996-01-01 |
| Language |
English |
| Type |
article |
| Abstract |
Five different laboratory tests for the identification of Staphylococcus
aureus were compared. Analyses of 271 presumptive S. aureus strains,
supplemented with 59 well-defined methicillin-resistant S. aureus (MRSA)
isolates, were performed. Only the Staphaurex Plus (Murex Diagnostics,
Dartford, United Kingdom) and the Pastorex Staphplus (Sanofi,
Marnes-La-Coquette, France) tests displayed 100% sensitivity. The observed
difference with the free-coagulase test (Bacto coagulase plasma; Difco,
Detroit, Mich.), a bound-coagulase (clumping factor) test, and the former
Staphaurex test (Murex Diagnostics) was caused mainly by the inability of
these three tests to identify some MRSA strains correctly. Among Polish
MRSA isolates included in the analysis, a group of free-coagulase-negative
S. aureus strains was detected. Genetic typing by random amplification of
polymorphic DNA revealed that the strains showing aberrant behavior when
the different test results were compared belonged to limited number of S.
aureus clones. |
| Publication |
http://hdl.handle.net/1765/8630 |
| Persistent Identifier |
urn:NBN:nl:ui:15-1765/8630 |
| Metadata |
XML |
| Repository |
Erasmus University Rotterdam |