| Titel | VIS2FIX: A High-Speed Fixation Method for Immuno-Electron Microscopy |
|---|---|
| Gepubliceerd in | Traffic, Vol. 12, p.806-814. ISSN 1398-9219. |
| Auteur | Karreman, M.A.; Donselaar, E.G. van; Gerritsen, H.C.; Verrips, C.T.; Verkleij, A.J. |
| Datum | 2011 |
| Trefwoord(en) | correlative microscopy, high-pressure freezing, HUVEC, iLEM, immuno-transmission electron microscopy, lipid, MDCK-II, section fixation, THP-1, VIS2FIX |
| Taal | Engels |
| Type | artikel |
| Uitgever | John Wiley and Sons |
| Samenvatting | Immuno-transmission electron microscopy (TEM) is the technique of choice for high-resolution localization of proteins in fixed specimen. Here we introduce 2 novel methods for the fixation of sections from cryo-immobilized samples that result in excellent ultrastructural preservation. These high-speed fixation techniques, both called VIS2FIX, allow for a reduction in sample preparation time from at least 1 week to only 8 h. The methods were validated in immuno-TEM experiments on THP-1 monocytes, human umbilical vein endothelial cells (HUVECs) and Madin–Darby canine kidney (MDCK-II) cells. The fixation and retention of neutral lipids is demonstrated, offering unique prospects for the application of immuno-TEM in the lipidomics field. Furthermore, the VIS2FIX methods were successfully employed in correlative fluorescence and electron microscopy. |
| Publicatie | http://igitur-archive.library.uu.nl/phys/2012-0206-200254/UU... |
| OpenURL | Zoek deze publicatie in (uw) bibliotheek |
| Persistent Identifier | URN:NBN:NL:UI:10-1874-224507 |
| Metadata | XML |
| Repository | Universiteit Utrecht |
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