| Title | Comprehensive two-dimensional liquid chromatography with ultraviolet, evaporative light scattering and mass spectrometric detection of triacylglycerols in corn oil |
|---|---|
| Published in | Journal of Chromatography. A, Including electrophoresis and other separation methods, Vol. 1178, No. 1-2, p.43-55. ISSN 0021-9673. |
| Author | Klift, van der E.J.C.; Vivó-Truyols, G.; Claassen, F.W.; Holthoon, van F.L.; Beek, van T.A. |
| Date | 2008 |
| Language | English |
| Type | article |
| Abstract | An improved comprehensive two-dimensional (LC × LC) HPLC system for the analysis of triacylglycerols was developed. In the first-dimension, a Ag(I)-coated cation exchanger (250 mm × 2.1 mm, 5 ¿m) was employed with a gradient from 100% MeOH to 6% MeCN in MeOH at 20 ¿L/min. Using a 10-way valve with two switching loops, 1 min sections of the first-dimension were introduced in the second-dimension consisting of a 30 mm × 4.6 mm C18 (1.8 ¿m) column with an isocratic mobile phase of methanol¿methyl tert-butyl ether (70:30) at 3.0 mL/min. As the second-dimension solvent was stronger than the first-dimension solvent, focusing in the second-dimension took place, leading to better separations than in previously reported analyses in which hexane was the main constituent of the first-dimension eluent. Compounds differing by 2 in their partition number were baseline separated in the second-dimension. Detection took place by UV at 210 nm, evaporative light scattering and (+)-atmospheric pressure chemical ionisation-MS with the latter giving the best results. Corn oil was investigated and 44 compounds could be detected: 34 triacylglycerols (TAGs), 8 oxygenated TAGs, and 2 TAGs containing a trans double bond. Data manipulation allowed the construction of contour plots and the automated calculation of the first- and second-dimension retention times and peak areas. Quantitative results are compared with a fatty acid methyl ester analysis, and with literature data. |
| Publication | http://library.wur.nl/WebQuery/wurpubs/360573 |
| OpenURL | Search this publication in (your) library |
| Persistent Identifier | urn:nbn:nl:ui:32-360573 |
| Metadata | XML |
| Repository | Wageningen University & Research Centre |
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