Comprehensive two-dimensional liquid chromatography with ultraviolet,... (2008)

Title Comprehensive two-dimensional liquid chromatography with ultraviolet, evaporative light scattering and mass spectrometric detection of triacylglycerols in corn oil
Published in Journal of Chromatography. A, Including electrophoresis and other separation methods, Vol. 1178, No. 1-2, p.43-55. ISSN 0021-9673.
Author Klift, van der E.J.C.; Vivó-Truyols, G.; Claassen, F.W.; Holthoon, van F.L.; Beek, van T.A.
Date 2008
Reference(s) Laboratorium voor Organische chemie, Extern overig, Laboratory for Organic Chemistry, Extern other
Language English
Type Article
Abstract An improved comprehensive two-dimensional (LC × LC) HPLC system for the analysis of triacylglycerols was developed. In the first-dimension, a Ag(I)-coated cation exchanger (250 mm × 2.1 mm, 5 ¿m) was employed with a gradient from 100% MeOH to 6% MeCN in MeOH at 20 ¿L/min. Using a 10-way valve with two switching loops, 1 min sections of the first-dimension were introduced in the second-dimension consisting of a 30 mm × 4.6 mm C18 (1.8 ¿m) column with an isocratic mobile phase of methanol¿methyl tert-butyl ether (70:30) at 3.0 mL/min. As the second-dimension solvent was stronger than the first-dimension solvent, focusing in the second-dimension took place, leading to better separations than in previously reported analyses in which hexane was the main constituent of the first-dimension eluent. Compounds differing by 2 in their partition number were baseline separated in the second-dimension. Detection took place by UV at 210 nm, evaporative light scattering and (+)-atmospheric pressure chemical ionisation-MS with the latter giving the best results. Corn oil was investigated and 44 compounds could be detected: 34 triacylglycerols (TAGs), 8 oxygenated TAGs, and 2 TAGs containing a trans double bond. Data manipulation allowed the construction of contour plots and the automated calculation of the first- and second-dimension retention times and peak areas. Quantitative results are compared with a fatty acid methyl ester analysis, and with literature data.
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Persistent Identifier urn:nbn:nl:ui:32-360573
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Repository Wageningen University & Research Centre

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