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Identification of in vitro phosphorylation sites in the Arabidopsis... (2009)

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Titel Identification of in vitro phosphorylation sites in the Arabidopsis thaliana somatic embryogenesis receptor-like kinase
Gepubliceerd in Proteomics, Vol. 9, No. 2, p.368-379. ISSN 1615-9853.
Auteur Karlova, R.B.; Boeren, J.A.; Kwaaitaal, M.A.C.J.; Aker, J.C.M.; Vervoort, J.J.M.; Vries, de S.C.
Datum 2009
Trefwoord(en) Laboratorium voor Biochemie
Taal Engels
Type Artikel
Samenvatting The Arabidopsis thaliana somatic embryogenesis receptor-like kinase (SERK) family consists of five leucine-rich repeat receptor-like kinases (LRR-RLKs) with diverse functions such as brassinosteroid insensitive 1 (BRI1)-mediated brassinosteroid perception, development and innate immunity. The autophosphorylation activity of the kinase domains of the five SERK proteins was compared and the phosphorylated residues were identified by LC-MS/MS. Differences in autophosphorylation that ranged from high activity of SERK1, intermediate activities for SERK2 and SERK3 to low activity for SERK5 were noted. In the SERK1 kinase the C-terminally located residue Ser-562 controls full autophosphorylation activity. Activation loop phosphorylation, including that of residue Thr-462 previously shown to be required for SERK1 kinase activity, was not affected. In vivo SERK1 phosphorylation was induced by brassinosteroids. Immunoprecipitation of CFP-tagged SERK1 from plant extracts followed by MS/MS identified Ser-303, Thr-337, Thr-459, Thr-462, Thr-463, Thr-468, and Ser-612 or Thr-613 or Tyr-614 as in vivo phosphorylation sites of SERK1. Transphosphorylation of SERK1 by the kinase domain of the main brassinosteroid receptor BRI1 occurred only on Ser-299 and Thr-462. This suggests both intra- and intermolecular control of SERK1 kinase activity. Conversely, BRI1 was transphosphorylated by the kinase domain of SERK1 on Ser-887. BRI1 kinase activity was not required for interaction with the SERK1 receptor in a pull down assay
Publicatie http://library.wur.nl/WebQuery/wurpubs/373200
OpenURL Zoek deze publicatie in (uw) bibliotheek
Persistent Identifier urn:nbn:nl:ui:32-373200
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Repository Wageningen Universiteit & Researchcentrum

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