| Title | Untargeted LC-Q-TOF mass spectrometry method for the detection of adulterations in skimmed-milk powder |
|---|---|
| Published in | Journal of Separation Science, Vol. 32, No. 8, p.1216-1223. ISSN 1615-9306. |
| Author | Cordewener, J.H.G.; Luykx, D.M.A.M.; Frankhuizen, R.; Bremer, M.G.E.G.; Hooijerink, H.; America, A.H.P. |
| Date | 2009 |
| Language | English |
| Type | article |
| Abstract | A nontargeted protein identification method was developed to screen for adulterations in skimmed-milk powder (SMP). There are indications of falsified SMP content due to the addition of plant proteins. To demonstrate the reliability and accuracy of the developed comparative LC-MS method using a quadrupole TOF MS instrument, adulterated SMP samples were prepared by the addition of protein isolates of soy and pea to skimmed-milk before pasteurisation and evaporation. The comparative LC-MS approach enabled unequivocal discrimination of those SMP samples containing soy and pea protein from nonadulterated SMP. To identify the source of (plant) proteins present in the adulterated SMP, data-dependent LC-MS/MS was used in combination with an include list of differential peptides. Numerous peptides originating from the major seed proteins of soy (glycinin, beta-conglycin) and pea (legumin, vicilin) could be identified in this way |
| Publication | http://library.wur.nl/WebQuery/wurpubs/379988 |
| OpenURL | Search this publication in (your) library |
| Persistent Identifier | urn:nbn:nl:ui:32-379988 |
| Metadata | XML |
| Repository | Wageningen University & Research Centre |
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