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Growth of oil accumulating microalga Neochloris oleoabundans under... (2012)

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Title Growth of oil accumulating microalga Neochloris oleoabundans under alkaline-saline conditions
Published in Bioresource Technology, Vol. 104, p.593-599. ISSN 0960-8524.
Author Santos, A.M.; Janssen, M.G.J.; Lamers, P.P.; Evers, W.A.C.; Wijffels, R.H.
Date 2012
Reference(s) Leerstoelgroep Bioprocestechnologie
Language English
Type Article
Abstract The effect of elevated pH and salt concentration on the growth of the freshwater microalga Neochloris oleoabundans was investigated. A study was conducted in 24-well plates on the design of a growth medium and subsequently applied in a photobioreactor. An artificial seawater medium with reduced Ca(2+) and PO(4)(3-) could prevent mineral precipitation at high pH levels. Growth was characterized in this new medium at pH8.1 and at pH10.0, with 420mM of total salts. Specific growth rates of 0.08h(-1) at pH8.1 and 0.04h(-1) at pH10.0 were obtained under controlled turbidostat cultivation. The effect of nitrogen starvation on lipid accumulation was also investigated. Fatty acids content increased not only with nitrogen limitation but also with a pH increase (up to 35% in the dry biomass). Fluorescence microscopy gave visual proof that N. oleoabundans accumulates oil bodies when growing in saline conditions at high pH.
Publication http://library.wur.nl/WebQuery/wurpubs/416332
OpenURL Search this publication in (your) library
Persistent Identifier urn:nbn:nl:ui:32-416332
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Repository Wageningen University & Research Centre

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