| Titel | Growth of oil accumulating microalga Neochloris oleoabundans under alkaline-saline conditions |
|---|---|
| Gepubliceerd in | Bioresource Technology, Vol. 104, p.593-599. ISSN 0960-8524. |
| Auteur | Santos, A.M.; Janssen, M.G.J.; Lamers, P.P.; Evers, W.A.C.; Wijffels, R.H. |
| Datum | 2012 |
| Trefwoord(en) | Leerstoelgroep Bioprocestechnologie |
| Taal | Engels |
| Type | artikel |
| Samenvatting | The effect of elevated pH and salt concentration on the growth of the freshwater microalga Neochloris oleoabundans was investigated. A study was conducted in 24-well plates on the design of a growth medium and subsequently applied in a photobioreactor. An artificial seawater medium with reduced Ca(2+) and PO(4)(3-) could prevent mineral precipitation at high pH levels. Growth was characterized in this new medium at pH8.1 and at pH10.0, with 420mM of total salts. Specific growth rates of 0.08h(-1) at pH8.1 and 0.04h(-1) at pH10.0 were obtained under controlled turbidostat cultivation. The effect of nitrogen starvation on lipid accumulation was also investigated. Fatty acids content increased not only with nitrogen limitation but also with a pH increase (up to 35% in the dry biomass). Fluorescence microscopy gave visual proof that N. oleoabundans accumulates oil bodies when growing in saline conditions at high pH. |
| Publicatie | http://library.wur.nl/WebQuery/wurpubs/416332 |
| OpenURL | Zoek deze publicatie in (uw) bibliotheek |
| Persistent Identifier | urn:nbn:nl:ui:32-416332 |
| Metadata | XML |
| Repository | Wageningen Universiteit & Researchcentrum |
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