Surface charge-specific cytotoxicity and cellular uptake of tri-block... (2013)

Title Surface charge-specific cytotoxicity and cellular uptake of tri-block copolymer nanoparticles
Published in Nanotoxicology, Vol. 7, No. 1, p.71-84. ISSN 1743-5390.
Author Bhattacharjee, S.; Ershov, D.S.; Gucht, van der J.; Alink, G.M.; Rietjens, I.; Zuilhof, H.; Marcelis, A.T.M.
Date 2013
Reference(s) Sub-department of Toxicology, Laboratorium voor Organische chemie, Laboratorium voor Fysische chemie en kolloïdkunde, Sub-department of Toxicology, Laboratory for Organic Chemistry, Physical Chemistry and Colloid Science
Language English
Type Article
Abstract A series of monodisperse (45 ± 5 nm) fluorescent nanoparticles from tri-block copolymers (polymeric nanoparticles (PNPs)) bearing different surface charges were synthesised and investigated for cytotoxicity in NR8383 and Caco-2 cells. The positive PNPs were more cytotoxic and induced a higher intracellular reactive oxygen species production than the neutral and negative ones. The cytotoxicity of positive PNPs with quaternary ammonium groups decreased with increasing steric bulk. The intracellular uptake and cellular interactions of these different PNPs were also tested in NR8383 cells by confocal laser scanning microscopy, which revealed higher uptake for positive than for negative PNPs. Also positive PNPs were found to interact much more with cell membranes, whereas the negative PNPs were internalised mainly by lysosomal endocytosis. Uptake of positive PNPs decreased with increasing steric bulk around the positive charge. A surface charge-specific interaction of clathrin for positive PNPs and caveolin receptors for negative PNPs was observed. These findings confirm that surface charge is important for the cytotoxicity of these PNPs, while they additionally point to considerable additional effects of the steric shielding around positive charges on PNP cytotoxicity.
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Persistent Identifier urn:nbn:nl:ui:32-421480
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Repository Wageningen University & Research Centre

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