| Title | A new ultrafast and high-throughput mass spectrometric approach for the therapeutic drug monitoring of the multi-targeted anti-folate pemetrexed in plasma from lung cancer patients |
|---|---|
| Published in | Analytical and Bioanalytical Chemistry, Vol. 398, No. 7-8, p.2943-2948. ISSN 1618-2642. |
| Author | Meesters, R.J.W. (Roland); Cornelissen, R. (Robin); Klaveren, van R.J. (Rob); Jonge, de R. (Robert); Boer, den E. (Ethan); Lindemans, J. (Jan); Luider, T.M. (Theo) |
| Date | 2010-12-01 |
| Language | English |
| Type | article |
| Abstract | An analytical assay has been developed and validated for ultrafast and high-throughput mass spectrometric determination of pemetrexed concentrations in plasma using matrix assisted laser desorption/ionization-triple quadrupole-tandem mass spectrometry. Patient plasma samples spiked with the internal standard methotrexate were measured by multiple reaction monitoring. The detection limit was 0.4 fmol/μL, lower limit of quantification was 0.9 fmol/μL, and upper limit of quantification was 60 fmol/μL, respectively. Overall observed pemetrexed concentrations in patient samples ranged between 8.7 (1.4) and 142.7 (20.3)∈pmol/μL (SD). The newly developed mass spectrometric assay is applicable for (routine) therapeutic drug monitoring of pemetrexed concentrations in plasma from non-small cell lung cancer patients. |
| Publication | http://hdl.handle.net/1765/28616 |
| Persistent Identifier | urn:NBN:nl:ui:15-1765/28616 |
| Metadata | XML |
| Repository | Erasmus University Rotterdam |
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