Research is centered towards some fundamental mechanisms which determine the development of disease specific gene therapies. The diseases under study are: cystic fibrosis, thalassaemia, immunodeficiencies, Crigler Najjar, neoplasia and restenosis. The present and future research is focused on the development of non-viral delivery vehicles with particular attention to the process of the transport of DNA into the nucleus in non-dividing cells and the integration of DNA into the host genome. In addition we are developing replicating vectors based on papilloma virus replication origins. Part of this project is a joint effort with the departments of Pediatrics (ZKL 540207), Cardiology (TOC 430604) and Surgery (HKG 470101).