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Urolithiasis in al zijn preklinische en klinische aspecten

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Titel Urolithiasis in al zijn preklinische en klinische aspecten
Looptijd 01 / 1988 - onbekend
Status Afgesloten
Onderzoeknummer OND1281242
Leverancier gegevens METIS Erasmus Universiteit Rotterdam

Samenvatting (EN)

Kidney stones occur frequently and may affect 10 to 15 % of the population in developed countries. The majority of renal stones are composed predominantly of calcium oxalate (CaOx) and the incidence of this stone type has climbed steadily in recent decades. After treatment, about half of all first stone formers will form kidney stones again. The stones are composed of myriad crystals cemented with organic material. The formation of crystals in the kidney is normal, but their retention in this organ is not. The attachment of crystals to the epithelial cells lining the renal tubules could play a crucial role in this pathological process. Whereas CaOx crystals cannot adhere to an intact epithelium, these anti-adherence properties are lost during wound healing. Studies performed in cell culture as well as in animals demonstrated that injured/regenerating cells express the polysaccharide hyaluronan (HA), the glycoprotein osteopontin (OPN) and the transmembrane protein CD44 at the luminal surface while cells without affinity for crystals do not. Although the role of CD44 and OPN is not clear, HA has been identified as major crystal binding molecule. Interestingly, CD44 is a cell surface receptor for HA as well as for OPN. Our aim is to elucidate the interrelationship between CD44, HA and OPN in crystal binding during renal tubular injury and regeneration. We expect to accomplish this goal by studies performed in two distal tubule/collecting duct cell lines (MDCK strain I and RCCD1), in primary cultures of human renal tubular cells, in OPN knockout mice, in rats and in humans

Betrokken organisaties

Betrokken personen

Projectleider N.P.N. Buchholz
Projectleider Dr. D.J. Kok
Projectleider Dr. J.C. Romijn
Projectleider Prof.dr. F.H. Schröder

Classificatie

A70000 Volksgezondheid en gezondheidszorg
D21500 Histologie, celbiologie
D23220 Inwendige geneeskunde

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