Mucosal Immune Regulation in Early Infancy: Monitoring and Intervention


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Title Mucosal Immune Regulation in Early Infancy: Monitoring and Intervention
Period 01 / 2004 - 12 / 2011
Status Completed
Dissertation Yes
Research number OND1296092
Data Supplier Projectleider


Cow's milk allergy (CMA) is the most frequently diagnosed food allergy in infancy. In general, patients have a good prognosis because the majority acquire tolerance within the first years. Interventions have been proposed to accelerate tolerance and reduce morbidity. Probiotic supplementation could be effective through modulation of the immune system. We sought to determine whether supplementation with a combination of probiotics (Lactobacillus casei CRL431 and Bifidobacterium lactis Bb-12) accelerates tolerance to cow's milk (CM) in infants with CMA. METHODS: We performed a double-blind, randomized, placebo-controlled trial in 119 infants with CMA. Infants received CRL431 and Bb-12 supplemented to their standard treatment of extensively hydrolyzed formula for 12 months. Primary outcome was clinical tolerance to CM at 6 and 12 months of treatment. Furthermore, we analyzed T- and B-lymphocyte subsets (CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), and CD20(+)) in peripheral blood at randomization and at 12 months with flow cytometry and examined the presence of viable probiotic strains in fecal samples.

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Supervisor Prof.dr. J.C. de Jongste
Supervisor Prof.dr. E.E.S. Nieuwenhuis
Co-supervisor Dr. E.H.G. van Leer
Co-supervisor Dr. J.N. Samsom
Doctoral/PhD student Dr. J. Hol


D23362 Pediatrics

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