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Production of subtilases from hyperthermophilic microorganisms: a feasibility study

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Titel Production of subtilases from hyperthermophilic microorganisms: a feasibility study
Looptijd 10 / 1997 - 01 / 2004
Status Afgesloten
Dissertatie Ja
URL http://library.wur.nl/wda/abstracts/ab3518.html
URL http://library.wur.nl/wda/dissertations/dis3518.pdf
Onderzoeknummer OND1309791

Samenvatting (EN)

Microorganisms that are able to grow at high temperatures are called thermophiles (>55°C) or hyperthermophiles (>80°C). Growth at high temperatures involves thermostable enzymes, and the question how this stabilization takes place at a structural level has played a central role in research of these organisms. In addition, the interest in applying them in industry as biocatalysts has largely increased. The bacterial domain holds a few (hyper)thermophilic members, and the organisms that were used in this study, Fervidobacterium pennivorans, F. gondwanense and Thermotoga maritima, belong to the Thermotogales order. They grow optimally from 65 to 80°C and are able to use a large variety of carbohydrates and peptides, for which they possess a range of enzymes. This research describes the detailed molecular and biochemical characterization of enzymes that enable growth of these organisms on, occasionally, remarkable substrates like pectin or chicken feathers. The study on these so-called thermozymes is an example of an integrated approach in which bioinformatics, molecular genetics, structural analysis, DNA microarray analysis, biochemistry and fermentation has been combined. Keyword(s): THERMOPHILIC BACTERIA / ENZYME ACTIVITY / PROTEIN METABOLISM / CARBOHYDRATE METABOLISM / PECTATE LYASE / GLUCOSE-6-PHOSPHATE ISOMERASE

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A20000 Plantaardige productie en dierlijke productie
C10000 Biotechnologie
D22100 Microbiologie

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