Generation of a conditional arginase I knockout mouse to study the role of arginine in macrophages during chronic inflammatory disease
02 / 2003 - 02 / 2007
Aim: to establish the exact role of arginase in chronic inflammatory disease, the best way is to produce a knockout mouse. Intracellular arginine availability can be increased by decreasing the expression of the arginine degrading enzyme arginase. This enzyme is vital for postnatal survival because the classical knockout dies within 14 days after birth. We are generating a conditional knockout ouse of arginase. By deleting the enzyme arginase I in macrophages, we would like to investigate if arginase I is an effector or a bystander molecule in chronic inflammatory diseases.