In this project the biodiversity and detection of clinical important yeasts is investigated. Emphasis is on Malassezia spp., Trichosporon spp., Cryptococcus spp. (incl. C. neoformans), and Candida species. Next to biodiversity and population studies, the development of rapid and reliable identification tools is also important. The biodiversity of these yeasts is being studied using rDNA and other sequences, as well as DNA fingerprint approaches such as amplified fragment length polymorphism (AFLP). The genomes of several of these yeasts have been sequenced (e.g. Candida spp., Cryptococcus neoformans), which will allow the development of rapid and reliable molecular identification techniques.