Depending on the protein level, diets deficient in amino acids (AAs) have to be supplemented with free AAs to meet individual AAs requirements. Different studies have suggested that dietary AAs in free form are less well utilized than protein-bound AAs. In general it is assumed that segregation of absorption through the digestive tract causes fractionation between free and bound AAs of the same meal. Both fractions will show increased oxidative losses of AAs due to a time lag between individual AAs and imbalanced protein. In this project the aim is to examine free AAs supplementation strategies on postprandial utilization of AAs and body composition of rats fed protein deficient diets.
[Results]:
1). Interaction between oxidative losses of endogenous and exogenous amino acids has been shown using 13CO2 breath test technique.
2). 1h time interval between methionine deficient meal and its free methionine supplement decreased oxidative losses of egg white boudn 1-13C leucine,
3). Diet 34% methionine defiecient fed to growing rats did not influence body weight gain.
4). Diet 45% lysine deficient fed to growing rats has strong influence on body weight gain,
5). The results showed an interaction between Lysine and Methionine in terms of influence on body weight gain and carcass composition of growing rats. |
