| MicroRNAs (miRNAs) are small (~21-23 nucleotide), non-protein-coding RNAs that mediate repression of messenger RNA (mRNA) translation or breakdown by base pairing to the 3' untranslated region of target messenger RNAs. A requirement for miRNAs in cellular differentiation is well established, but the identity of specific miRNAs in developmental processes remains unknown. We intend to use the mammalian hematopoietic system and novel miRNA libraries to elucidate specific miRNA function in differentiation. Using conditional Dicer-deficient mice, we have determined a requirement for miRNAs in the development of hematopoietic lineages. To unravel which miRNA(s) play a role in hematopoiesis, we will develop and exploit lentiviral libraries that allow gain-of-function screens by ectopic miRNA expression in Dicer-deficient cells. This fellowship experience will allow me to conduct research on noncoding RNAs, which will both broaden my insight and complement my expertise in a field where I hope to establish myself as an independent research scientist. Dissecting the role of miRNAs in hematopoiesis will contribute to our knowledge on mechanisms that control blood cell formation and may identify new factors that could be exploited in the development of novel therapeutic drugs. |
