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Signalling through Arabidopsis AtSERK LRR receptors; ligandidentification...

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Title Signalling through Arabidopsis AtSERK LRR receptors; ligandidentification and functional genomics.
Period 08 / 2003 - unknown
Status Completed
Dissertation Yes
URL http://edepot.wur.nl/16358
Research number OND1325107

Abstract

Communication between cells and between the organism and the environment is essential for a multicellular organism. This requires a multitude of signalling pathways as indicated by the predicted existence of several hundreds of transmembrane receptor kinases in the Arabidopsis thaliana genome. Only a very small number of recptor - ligand combinations have been determined. This project is focused on identification of the ligand for the Arabidopsis thaliana Somatic Embryogenesis Receptor Kinase 1 (AtSERK) gene. The AtSERK1 gene belongs to a small family of 5 related genes that is a part of the larger super family of leucin-rich-repeat (LRR)-containing serine- threonine receptor kinases. Ligands will be identified with the aid of an in vivo receptor-activation assay. It will also be determined whether such ligands belong to the group of classical growth regulators in plants such as auxin or cytokinin. The second aim is to use a biochemical and cell biological approach to determine the composition of the AtSERK signalling complexes in living cells. For this will make use of both immunocytological techniques and fluorescently labeled receptors. Using this combined approach, we will be able to visualize the dynamics in living cells of receptor activation and interaction with other proteins of the AtSERK signalling complex.

Abstract (NL)

Communicatie tussen cellen en tussen organismen en hun omgeving is essentieel voor het functioneren van een multicellulair organisme. Dit vereist de aanwezigheid van een groot aantal signaaltransductie routes zoals voorspeld door de aanwezigheid van enkele honderden verschillende receptor kinase genen in het Arabidopsis genoom. Van slechts enkele receptor kinases is bekend door welk ligand ze geactiveerd worden . Dit project is gericht op het identificeren van de liganden die receptoren van de kinase van Arabidopsis thaliana Somatic Embryogenesis Receptor Kinase (AtSERK) activeren. Deze familie van vijf recptoren, met als eerste lid het AtSERK 1 gen behoort tot de groteren superfamilie van leucin-rich-repeat (LRR)- bevattende serine-theorine receptor kinases. Met behulp van specifieke antilichamen gericht tegen de geactiveerde receptor en fluorescent gelabelde AtSERK receptors en eiwitten uit het AtSERK complex is het uiteindelijke doel om de dynamiek van receptor- activatie te vervolgen in levende cellen.

Related organisations

Related people

Supervisor Prof.dr. S.C. de Vries
Doctoral/PhD student Drs. J.C.M. Aker

Related research (upper level)

Classification

A21000 Agriculture and horticulture
C10000 Biotechnology
D21300 Biochemistry
D22100 Microbiology

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