| Microtubules are highly dynamic cytoskeletal elements, required for a variety of cellular functions. Recently, a family of microtubule plus end tracking proteins (+TIPs) was identified that specifically associates with the ends of growing microtubules in non-neuronal cells. We are interested in determining the mechanistic roles that these proteins play in the regulation of microtubule dynamic events. It is generally accepted that the architectural organisation of the nucleus and regulation of transcription are functionally linked. We are interested in describing the constituents of nuclear and nucleolar chromatin and in the phenomenon of imprinting. We have chosen to generate gene knock outs of several multi-zinc finger transcription factors, which have been implicated in chromatin organisation and maintenance of chromatin domains. |
