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Processing and Assembly of rRNA

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Title Processing and Assembly of rRNA
Period 01 / 2008 - unknown
Status Completed
Research number OND1328729
Data Supplier Website IMBW

Abstract

In the yeast Saccharomyces cerevisiae, as in in most other eukaryotic cells, the 18S, 5.8S and 25S rRNAs are transcribed by RNA polymerase I as a single precursor molecule containing both internal (ITS1 and ITS2) and external (5'ETS and 3'ETS) transcribed spacers, which are subsequently removed in an ordered series of endo- and exonucleolytic cleavages. While the position of these cleavages and the order in which they occur is now well-established our knowledge of the molecular mechanisms underlying rRNA maturation and the manner in which pre-rRNA processing is linked to assembly with the ribosomal proteins is still very limited. Over the past years it has become clear, however, that pre-rRNA maturation and assembly are highly complex processes both guided by structural elements within the precursor (cis-acting elements) and requiring the participation of numerous trans-acting (non-ribosomal) factors, whose precise role remains largely to be established. Our current interest focuses on several of these trans-acting factors. In particular we are studying Rrp5p, one of only two proteins known to be required for biogenesis of both the large and small ribosomal subunit; Rio2p, a novel type of protein kinase, that is essential for the final, cytoplasmic step in small subunit maturation and Rrp9p, a component of the U3 snoRNP particle that initiates the formation of a large processing complex on the primary pre-rRNA transcript. In this research we employ a wide array of genetic (e.g. suppressor analysis, synthetic lethality ), molecular-biological and biochemical techniques.

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Project leader Prof.dr. H.A. Raué
Project leader Dr. J.C. Vos

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