Plant parasitic nematodes cause considerable damage to crops. Knowledge about the presence of nematodes in a field allows the grower to take control measures. There is a need for a fast, reliable test allowing the quantitative, simultaneous detection of multiple nematode species in a sample. Existing multiplex detection tests are not suitable for the quantitative detection of so many different species.
Research objectives:
The Biotrove Open Array system enables quantitative detection of multiple species in a single experiment using low reaction volumes (saving costs for expensive ingredients) and with limited pipetting steps (saving labour costs). It requires the availability of suitable real-time PCRs. Experiments performed in 2007 and 2008 have shown that TaqMan PCRs and SyberGreen PCRs can be for the detection of nematodes when performed on the Biotrove system.
In this project we will investigate which of the real-time PCRs are most suitable with respect to sensitivity and quantification.
Results and products:
Intended results are:
* Information regarding suitability of real-time PCRs for detection of nematodes extracted from soil samples, with respect to sensitivity, level of quantification and reproducibility
* Information about the number of samples that can be analyzed at the same time using one Biotrove array when detecting 2, 3, 4 to 8 differrent nematode species in a quantitative manner
Intended product is a fast and reliable test for quantitative multiplex detection of different nematode species in soil samples. |
