Protein immobilisation and positioning in microchannels
02 / 2006 - 01 / 2012
Many processes, especially in biological systems, require enantio pure compounds. Particularly in the pharmaceutical and fine chemical industry enantioselective synthesis is therefore becoming more and more important. While impurities can give undesired properties to the product, qualitative and/or quantitative analysis will be mandatory. Nowadays synthetical research done with microreactor technology is increasing. Due to the low amounts of product and fast reaction time, conventional analysis methods like HPLC or GC-MS are not adequate to monitor these micro-scaled reactions online. For this reason a micro-scaled pressure driven system will be developed which will be applicable for online detection of the enantiomeric excess. In this project a monolithic affinity microcolumn for enantioselective analysis will be developed. A monolith is a polymeric porous network, which has low backpressures, making it very compatible with pressure driven systems. Chiral selectors, like proteins can be immobilized onto the monolithic structure to obtain a chiral column. By varying several factors like the chiral selectors, monolithic components, channel design e.g. a general applicable microcolumn could be derived.