Description:
For the development of a microarray biosensor immunoassay for hazard analysis of food chains several steps have to be made. Reproducable and high capacity antigen and antibody spots have to be made, the sensitivity of the detection system has to be established, and multi-analyte assays have to be developed and tested with real food matrices.
Research objectives:
This project aims at developing a multi-analyte screening device for hazard analysis of food chains. For this purpose a multi-immunoassay on top of an SPR- imaging platform in order to detect rapidly and simultaneously various food contaminants (e.g. food allergens, residues of veterinary drugs and environmental contaminants) will be developed.
So far, spotting systems are tested for the production of reproducible microarrays for use with a flow cell and a cuvette system. The limit of detection, sensitivity and robustness of the IBIS iSPR biosensor are determined and found to be satisfactory. The first multiplex for antibiotics is developed and was as sensitive and robust as in other SPR biosensors. Multiplex assays for high molecular weight compounds are being developed. At the end of the project associated companies will utilize marketable products. |
