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Molecular architecture of environmental adaptation in natural populations...

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Titel Molecular architecture of environmental adaptation in natural populations of the nematode Caenorhabditis elegans (NEMADAPT)
Looptijd 01 / 2010 - onbekend
Status Lopend
Onderzoeknummer OND1339429
Leverancier gegevens NWO

Samenvatting (EN)

Almost all species adapt their life-history or behaviour in the face of continuous environmental challenges. Two distinct adaptive responses can be favoured under these conditions: a phenotypic plastic response and an evolutionary response. In case of a phenotypic plastic response, selection favoured the ability of individuals to adapt by adjusting metabolic processes or specific behaviours. Adaptation is thus determined at the transcriptomic or proteomic level. In case of an evolutionary response, selection acts on the genetic composition of the population as a consequence of differential mortality and reproductive success. Adaptation is thus manifested at the DNA level. In both responses, the options for fitness maximization are constrained by trade-offs such as increasing stress resistance at the cost of offspring number. But so far it is unknown 1) which genes are the target of natural selection and how they contribute to phenotypic variation, and 2) how trade-offs are regulated in natural populations under different environmental regimes. We will address these questions by studying natural populations of C. elegans. It is a cosmopolitan nematode displaying high local genetic diversity, accessible to molecular high-throughput screens and allows for associations between genotype and phenotype to pinpoint the loci responsible for phenotypic variation. We will i) isolate 240 wildtype strains and their associated micro-organisms at 2 different locations and 2 distinct habitats, ii) measure SNP variation and the transcriptome in all strains, iii) measure phenotypic variation and trade-offs in response to the biotic and abiotic stressors, iv) search for candidate genes using association mapping, and v) validate these candidates using RNAi knock-downs. By taking advantage of the model C. elegans we are one of the first initiatives to combine phenotypic screens, association mapping and RNAi-mediated gene validation and unravel the molecular architecture of adaptation in natural populations.

Betrokken organisaties

Betrokken personen

Projectleider Dr.ir. J.E. Kammenga

Classificatie

A13000 Bodem
D22100 Microbiologie

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